Technology:
The technology we have today is the reason cloning can occur. Scientists are still developing new technology to avoid the certain issues regarding the process of cloning which will essentially make it safer and easier to use in the future.
Some of the equipment used in the lab for the process of cloning are listed below:
- Microscopes
- Robotic Arm to inject cells
- Computers for data analysis
- Inubators to store cells and let them grow
- Fluorescent Lights to test for GFP (we use it as a marker)
- PCR machines
- Gels for DNA electrophoresis and Western Blot
- Culture Hoods to keep cell lines sterile from other life
- Many freezers at -80C to store cells and some enzymes/DNA
- Refrigerator for keeping cell culture fluids (media, enzymes)
- Chemicals needed for cell culture
- Flow Cytometry units
- Mice/Rabbits for testing
The process of cloning is quite simple as their are only a few steps involved. Firstly DNA from the cell of an adult animal, called the "donor," is extracted from the cell and inserted into an egg cell from another animal. The egg cell has had its nucleus removed so that it will read and duplicate the DNA of the donor cell.The newly created embryo is then zapped with electricity so that it starts multiplying, until it becomes a blastocyst, which is then implanted into a surrogate mother. The resulting newborn will be an identical genetic replica to the donor animal.
Some of the equipment used in the lab for the process of cloning are listed below:
- Microscopes
- Robotic Arm to inject cells
- Computers for data analysis
- Inubators to store cells and let them grow
- Fluorescent Lights to test for GFP (we use it as a marker)
- PCR machines
- Gels for DNA electrophoresis and Western Blot
- Culture Hoods to keep cell lines sterile from other life
- Many freezers at -80C to store cells and some enzymes/DNA
- Refrigerator for keeping cell culture fluids (media, enzymes)
- Chemicals needed for cell culture
- Flow Cytometry units
- Mice/Rabbits for testing
The process of cloning is quite simple as their are only a few steps involved. Firstly DNA from the cell of an adult animal, called the "donor," is extracted from the cell and inserted into an egg cell from another animal. The egg cell has had its nucleus removed so that it will read and duplicate the DNA of the donor cell.The newly created embryo is then zapped with electricity so that it starts multiplying, until it becomes a blastocyst, which is then implanted into a surrogate mother. The resulting newborn will be an identical genetic replica to the donor animal.